basic parasite nucleofector kit 2 buffers and reagents Search Results


nucleofection positive control  (Thermo Fisher)
99
Thermo Fisher nucleofection positive control
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Nucleofection Positive Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
nucleofection positive control - by Bioz Stars, 2026-03
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human stem cell nucleofection buffer  (Lonza)
90
Lonza human stem cell nucleofection buffer
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Human Stem Cell Nucleofection Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofection buffer/product/Lonza
Average 90 stars, based on 1 article reviews
human stem cell nucleofection buffer - by Bioz Stars, 2026-03
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hesc nucleofector solution kit 2  (Amaxa)
90
Amaxa hesc nucleofector solution kit 2
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Hesc Nucleofector Solution Kit 2, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hesc nucleofector solution kit 2 - by Bioz Stars, 2026-03
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human stem cell nucleofector kit 2 solution  (Lonza)
90
Lonza human stem cell nucleofector kit 2 solution
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Human Stem Cell Nucleofector Kit 2 Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofector kit 2 solution/product/Lonza
Average 90 stars, based on 1 article reviews
human stem cell nucleofector kit 2 solution - by Bioz Stars, 2026-03
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basic parasite nucleofector kit 2  (Amaxa)
90
Amaxa basic parasite nucleofector kit 2
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Basic Parasite Nucleofector Kit 2, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
basic parasite nucleofector kit 2 - by Bioz Stars, 2026-03
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nucleofection buffer  (Lonza)
90
Lonza nucleofection buffer
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Nucleofection Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleofection buffer/product/Lonza
Average 90 stars, based on 1 article reviews
nucleofection buffer - by Bioz Stars, 2026-03
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amaxa basic ® parasite nucleofector kit 2 solution  (Lonza)
90
Lonza amaxa basic ® parasite nucleofector kit 2 solution
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Amaxa Basic ® Parasite Nucleofector Kit 2 Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
amaxa basic ® parasite nucleofector kit 2 solution - by Bioz Stars, 2026-03
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amaxa nucleoporator  (Amaxa)
90
Amaxa amaxa nucleoporator
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Amaxa Nucleoporator, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleoporator  (Amaxa)
90
Amaxa nucleoporator
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Nucleoporator, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleofection system  (Amaxa)
90
Amaxa nucleofection system
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Nucleofection System, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleofection system/product/Amaxa
Average 90 stars, based on 1 article reviews
nucleofection system - by Bioz Stars, 2026-03
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parasite nucleofector solution  (Lonza)
90
Lonza parasite nucleofector solution
Timeline for <t>nucleofection,</t> subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.
Parasite Nucleofector Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/parasite nucleofector solution/product/Lonza
Average 90 stars, based on 1 article reviews
parasite nucleofector solution - by Bioz Stars, 2026-03
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Image Search Results


Timeline for nucleofection, subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.

Journal: Current protocols in human genetics

Article Title: CRISPR/Cas9 Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells

doi: 10.1002/cphg.52

Figure Lengend Snippet: Timeline for nucleofection, subcloning, and expansion of fluorescently-tagged hiPSCs. Assume a daily mTeSR1 media change for all hiPSC steps. Rho kinase inhibitor is recommended for passaging and steps where hiPSCs are plated sparsely or as single cells. A) Steps from nucleofection until initial clone picking. Begin by nucleofecting 80% confluent, low passage hiPSCs and conduct puromycin selection. After selection, allow clones to grow before replating cells in a serial dilution at very low density. Then, identify individual cells under a microscope and allow single, monoclonal colonies to grow to 100 cells in rho kinase inhibitor before picking into a 96-well plate. B) Steps from clone picking to positive HDR clone identification. After repicking monoclonal hiPSC colonies into a new 96-well plate, allow the colonies to grow in rho kinase inhibitor before redistributing them within the same well. Then, allow colonies to into 95% confluent monolayers before passaging half into a new plate while simultaneously harvesting half for HDR verification with DNA extraction and PCR/gel electrophoresis. Once positive clones are identified, proceed to C. If no positive clones are identified, re-nucleofect with the same guide RNA or order new guide RNAs. C) Once positive clones are identified, grow the clones in 96-well plates and passage positive clones into 24 and 6-well plates, consecutively. After clones are grown to 6-well plate format, freeze a subset of clones before passaging and expanding for long-term culture.

Article Snippet: Materials Wild type, low passage hiPSCs adapted for feeder cell-free growth on Matrigel Matrigel (hESC-qualified; BD Sciences, cat. no. 354277) DMEM/F12 medium (Thermo Fisher Scientific, cat. no. 11320033) mTeSR1 medium (StemCell Technologies, cat. no. 05850) Rho kinase inhibitor (ROCKi; Tocris cat. no. 1254) Human Stem Cell Nucleofector Kit 2 (Lonza, cat. no. VPH-5022) GFP plasmid as nucleofection positive control (pMax GFP vector supplied with Human Stem Cell Nucleofector Kit 2) Cas9 plasmid DNA (from Basic Protocol 1) gRNA expression vector (from Basic Protocol 1) HDR template vector (from Basic Protocol 1) Phosphate-buffered saline (PBS; Life Technologies, cat. no. 20012-050) 0.5M EDTA (Thermo Fisher Scientific, cat. no. 15575020) 6-well tissue culture–treated plates 15- and 50-mL conical centrifuge tubes (e.g., BD Falcon) Hemocytometer for cell counting Tabletop centrifuge with plate adapter Amaxa Nucleofector II Device Transfect hiPSCs with CRISPR plasmids list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare 6-well plates coated with Matrigel extracellular matrix, on which hiPSCs will grow in a feeder cell-free format.

Techniques: Subcloning, Passaging, Selection, Clone Assay, Serial Dilution, Microscopy, DNA Extraction, Nucleic Acid Electrophoresis